Our super-resolution microscope has been up and running. Lasers: 532nm and 405nm (other colors are on the way).
Two samples were tested for super-resolution imaging:
- E. coli bacteria expressing H-NS proteins fused to mEoS3.2 (the strain was a gift from Dr. William Navarre and Dr. Joshua Milstein at the University of Toronto).
- E. coli bacteria expressing mEos3.2 only (from Addgene; the strain was a gift from Dr. Michael Davidson).
The same setup is also good for single-molecule experiments (FIONA, smFRET, etc).